FAQ



iMatrix-511, iMatrix-511 silk

Q1. What format(s) are the iMatrixTM products sold in?

A1. Currently, it is sold only in liquid form.
One tube contains 175 ug of cell culture substrate (laminin 511-E8 fragment) at a concentration of 0.5 mg / mL.

* The lyophilized version of iMatrixTM-511 has been discontinued by the manufacturer as of March 2015.

Q2. What is the product storage temperature and expiration date?

A2. The storage temperature of the product is refrigerated at 2-8 ° C.
Please refer to the table below for product expiration date.

Expiration date Applicable products
2 years from date of production iMatrixTM-511, iMatrixTM-511 silk, iMatrixTM-511MG, iMatrixTM-411, iMatrixTM-221
1 year and 6 months from date of production Easy iMatrixTM-511, Easy iMatrixTM-511 silk
6 months from date of production Quick iMatrixTM-511, Quick iMatrixTM-511 silk

[ The specific expiration date is printed on the product box. ]

Q3. Is it possible to store iMatrixTM frozen? (-80 °C)

A3. No. It can not be stored frozen.

Q4. What is the purity of the iMatrixTM products?

A4. Purity is over 95%. Please refer to the lot specific data sheet for more information.

Q5. What is the recommended medium for culturing hES/hiPS cells when using iMatrixTM products?

A5. Miyazaki et al. Nature communications, 3(1236), 1-10, 2012, and Scientific Reports, 7, 41165, 2017.
The following are used.
・mTeSR1,TeSR2,TeSR-E8 (STEMCELL Technologies)
・StemProhESCSFM (Thermo Fisher Scientific)
・StemFitAK03 (Ajinomoto)

Nakagawa et al. Scientific Reports, 4(3594), 1-7, 2014.
The following are used.
・StemFit (Ajinomoto)

The publication stated that all media showed good results.

Q6. What is the optimal coating concentration for iPS cell culture?

A6. The optimal coating concentration depends on the cell line and must be determined by the end user. We recommend first trying iMatrixTM at 0.5 ug / cm2 and consider the optimal concentration to be between 0.1 and 1.5 ug / cm2.

In addition, there is also a new method for plating, the Pre-mix method:
Miyazaki et al. Scientific Reports, 7, 41165, 2017.

Q7. Is there a culture protocol for iPS cells using iMatrixTM-511?

A7. Please refer to the link below for the expansion culture protocol of ES / iPS cells using iMatrixTM-511 and the movie of passage operation.
(Links to the external site.)
(Expansion culture protocol)
(Movie of passage operation)

Q8. Is Rock Inhibitor (Y-27632) necessary for culturing iPS cells?

A8. Nakagawa et al. Scientific Reports, 4(3594), 2014.
Use Rock Inhibitor at cell passage.
However, it is not used during medium exchange.

Q9. Can iPS cell passage be handled with a single cell?

A9. Yes. iPS cell passage can be handled with a single cell.
Nakagawa et al. Scientific Reports, 4(3594), 2014.

* Links to the external site.
(Expansion culture protocol)
(Movie of passage operation)

Q10. What is used to detached the cells?

A10. We recommend using trypsin.
Nakagawa et al. Scientific Reports, 4(3594), 2014.

* Links to the external site.
(Expansion culture protocol)
(Movie of passage operation)

Q11. Can this product be used for mouse iPS cells?

A11. Currently, there is no data to support culture of mouse iPS cells using iMatrixTM-511.

Q12. What is the difference between iMatrixTM-511 and Matrigel?

A12. Matrigel contains laminin-111 from mouse EHS sarcoma.
It also contains molecules other than laminin.
iMatrixTM-511 is a recombinant protein purified from laminin 511-E8 fragment expressed in CHO-S cells with high purity.
iMatrixTM-511 silk is a recombinant protein in which the laminin 511-E8 fragment is highly purified by silkworm-produced cocoons.
Human ES cells and iPS cells are known to adhere to laminin-511 through cell membrane receptors (especially a6b1 integrin).
Human ES cells and iPS cells are known to exhibit high adhesion activity to laminin-511.
This makes it possible to pass iPS cells into single cells and pass them with iMatrixTM-511 / iMatrixTM-511 silk.
In Miyazaki et al. (Nature communications, 3 (1236), 1-10, 2012), the expansion efficiency of the number of cells after 5 passages (after 30 days) is about 200 times higher than that of Matrigel.

Q13. Is it possible to purchase iMatrixTM-511 outside of Japan?

A13. Yes. It is possible to purchase iMatrixTM products from an overseas distributor.
For overseas distributor information, please refer to this page.
Overseas Distributor

iMatrix-411

Q1. What format(s) are the iMatrixTM products sold in?

A1. Currently, it is sold only in liquid form.
One tube contains 175 ug of cell culture substrate (laminin 511-E8 fragment) at a concentration of 0.5 mg / mL.

* The lyophilized version of iMatrixTM-511 has been discontinued by the manufacturer as of March 2015.

Q2. What is the product storage temperature and expiration date?

A2. The storage temperature of the product is refrigerated at 2-8 ° C.
Please refer to the table below for product expiration date.

Expiration date Applicable products
2 years from date of production iMatrixTM-511, iMatrixTM-511 silk, iMatrixTM-511MG, iMatrixTM-411, iMatrixTM-221
1 year and 6 months from date of production Easy iMatrixTM-511, Easy iMatrixTM-511 silk
6 months from date of production Quick iMatrixTM-511, Quick iMatrixTM-511 silk

[ The specific expiration date is printed on the product box. ]

Q3. What is the purity of the iMatrixTM products?/h3>
A3. No. It can not be stored frozen.

Q4. What is the purity of the iMatrixTM products?

A4. Purity is over 95%. Please refer to the lot specific data sheet for more information.

Q5. What is the recommended medium for culturing hES/hiPS cells when using iMatrixTM products?

A5. Miyazaki et al. Nature communications, 3(1236), 1-10, 2012, and Scientific Reports, 7, 41165, 2017.
The following are used.
・mTeSR1,TeSR2,TeSR-E8 (STEMCELL Technologies)
・StemProhESCSFM (Thermo Fisher Scientific)
・StemFitAK03 (Ajinomoto)

Nakagawa et al. Scientific Reports, 4(3594), 1-7, 2014.
The following are used.
・StemFit (Ajinomoto)

The publication stated that all media showed good results.

Q6. What is the optimal coating concentration for iPS cell culture?

A6. The optimal coating concentration depends on the cell line and must be determined by the end user. We recommend first trying iMatrixTM at 0.5 ug / cm2 and consider the optimal concentration to be between 0.1 and 1.5 ug / cm2.

In addition, there is also a new method for plating, the Pre-mix method:
Miyazaki et al. Scientific Reports, 7, 41165, 2017.

Q7. Is there a protocol to differentiate from iPS cells using iMatrixTM-411?

A7. Ohta et al. Scientific Reports, 6, 35680, 1-12, 2016.
In this paper, iPS cells are induced to differentiate into vascular endothelial cells.

Takayama et al. Biochemical and Biophysical Research Communications, 474, 1, 91-96, 2016.
This paper is inducing differentiation from iPS cells to cholangiocytes.

Q8. Can this product be used for mouse iPS cells?

A8. Currently, there is no data to support culture of mouse iPS cells using iMatrixTM-511.

Q9. What is the difference between iMatrixTM-511 and Matrigel?

A9. Matrigel contains laminin-111 from mouse EHS sarcoma.
It also contains molecules other than laminin.
iMatrixTM-511 is a recombinant protein purified from laminin 511-E8 fragment expressed in CHO-S cells with high purity.
iMatrixTM-511 silk is a recombinant protein in which the laminin 511-E8 fragment is highly purified by silkworm-produced cocoons.
Human ES cells and iPS cells are known to adhere to laminin-511 through cell membrane receptors (especially a6b1 integrin).
Human ES cells and iPS cells are known to exhibit high adhesion activity to laminin-511.
This makes it possible to pass iPS cells into single cells and pass them with iMatrixTM-511 / iMatrixTM-511 silk.
In Miyazaki et al. (Nature communications, 3 (1236), 1-10, 2012), the expansion efficiency of the number of cells after 5 passages (after 30 days) is about 200 times higher than that of Matrigel.

Easy iMatrix-511, Easy iMatrix-511silk

Q1. What format(s) are the iMatrixTM products sold in?

A1. Currently, it is sold only in liquid form.
One bottle contains 100 mL of cell culture substrate solution at a concentration of 1.6 ug / mL.

* The lyophilized version of iMatrixTM-511 has been discontinued by the manufacturer as of March 2015.

Q2. What is the product storage temperature and expiration date?

A2. The storage temperature of the product is refrigerated at 2-8 ° C.
Please refer to the table below for product expiration date.

Expiration date Applicable products
2 years from date of production iMatrixTM-511, iMatrixTM-511 silk, iMatrixTM-511MG, iMatrixTM-411, iMatrixTM-221
1 year and 6 months from date of production Easy iMatrixTM-511, Easy iMatrixTM-511 silk
6 months from date of production Quick iMatrixTM-511, Quick iMatrixTM-511 silk

[ The specific expiration date is printed on the product box. ]

Q3. What is the purity of the iMatrixTM products?/h3>
A3. No. It can not be stored frozen.

Q4. What is the recommended medium for culturing hES/hiPS cells when using iMatrixTM products?

A4. Miyazaki et al. Nature communications, 3(1236), 1-10, 2012, and Scientific Reports, 7, 41165, 2017.
The following are used.
・mTeSR1,TeSR2,TeSR-E8 (STEMCELL Technologies)
・StemProhESCSFM (Thermo Fisher Scientific)
・StemFitAK03 (Ajinomoto)

Nakagawa et al. Scientific Reports, 4(3594), 1-7, 2014.
The following are used.
・StemFit (Ajinomoto)

The publication stated that all media showed good results.

Q5. What is the optimal coating volume for iPS cell culture?

A5. 6-well plate;
Add 1.5 mL / well (9.6 cm2) of Easy iMatrixTM.
The coating concentration is 0.25 ug / cm2.

Q6. Is Rock Inhibitor (Y-27632) necessary for culturing iPS cells?

A6. Nakagawa et al. Scientific Reports, 4(3594), 2014.
Use Rock Inhibitor at cell passage.
However, it is not used during medium exchange.

Q7. Can iPS cell passage be handled with a single cell?

A7. Yes. iPS cell passage can be handled with a single cell.
Nakagawa et al. Scientific Reports, 4(3594), 2014.

* Links to the external site.
(Expansion culture protocol)
(Movie of passage operation)

Q8. What is used to detached the cells?

A8. We recommend using trypsin.
Nakagawa et al. Scientific Reports, 4(3594), 2014.

* Links to the external site.
(Expansion culture protocol)
(Movie of passage operation)

Q9. Can this product be used for mouse iPS cells?

A9. Currently, there is no data to support culture of mouse iPS cells using iMatrixTM-511.

Q10. What is the difference between iMatrixTM-511 and Matrigel?

A10. Matrigel contains laminin-111 from mouse EHS sarcoma.
It also contains molecules other than laminin.
iMatrixTM-511 is a recombinant protein purified from laminin 511-E8 fragment expressed in CHO-S cells with high purity.
iMatrixTM-511 silk is a recombinant protein in which the laminin 511-E8 fragment is highly purified by silkworm-produced cocoons.
Human ES cells and iPS cells are known to adhere to laminin-511 through cell membrane receptors (especially a6b1 integrin).
Human ES cells and iPS cells are known to exhibit high adhesion activity to laminin-511.
This makes it possible to pass iPS cells into single cells and pass them with iMatrixTM-511 / iMatrixTM-511 silk.
In Miyazaki et al. (Nature communications, 3 (1236), 1-10, 2012), the expansion efficiency of the number of cells after 5 passages (after 30 days) is about 200 times higher than that of Matrigel.

Quick iMatrix-511, Quick iMatrix-511silk

Q1. What format(s) are the iMatrixTM products sold in?

A1. They are sold as coated on culture plates.

The top half of the 6 well plate is the coating.
The lower half is non-coated.

Q2. What is the product storage temperature and expiration date?

A2. The storage temperature of the product is refrigerated at 2-8 ° C.
Please refer to the table below for product expiration date.

Expiration date Applicable products
2 years from date of production iMatrixTM-511, iMatrixTM-511 silk, iMatrixTM-511MG, iMatrixTM-411, iMatrixTM-221
1 year and 6 months from date of production Easy iMatrixTM-511, Easy iMatrixTM-511 silk
6 months from date of production Quick iMatrixTM-511, Quick iMatrixTM-511 silk

[ The specific expiration date is printed on the product box. ]

Q3. Is it possible to store iMatrixTM frozen? (-80 °C)

A3. No. It can not be stored frozen.

Q4. What is the recommended medium for culturing hES/hiPS cells when using iMatrixTM products?

A4. Miyazaki et al. Nature communications, 3(1236), 1-10, 2012, and Scientific Reports, 7, 41165, 2017.
The following are used.
・mTeSR1,TeSR2,TeSR-E8 (STEMCELL Technologies)
・StemProhESCSFM (Thermo Fisher Scientific)
・StemFitAK03 (Ajinomoto)

Nakagawa et al. Scientific Reports, 4(3594), 1-7, 2014.
The following are used.
・StemFit (Ajinomoto)

The publication stated that all media showed good results.

Q5. What is the coating concentration?

A5. One well: coated at a concentration of 0.25 ug / cm2.

Q6. Is Rock Inhibitor (Y-27632) necessary for culturing iPS cells?

A6. Nakagawa et al. Scientific Reports, 4(3594), 2014.
Use Rock Inhibitor at cell passage.
However, it is not used during medium exchange.

Q7. Can iPS cell passage be handled with a single cell?

A7. Yes. iPS cell passage can be handled with a single cell.
Nakagawa et al. Scientific Reports, 4(3594), 2014.

* Links to the external site.
(Expansion culture protocol)
(Movie of passage operation)

Q8. What is used to detached the cells?

A8. We recommend using trypsin.
Nakagawa et al. Scientific Reports, 4(3594), 2014.

* Links to the external site.
(Expansion culture protocol)
(Movie of passage operation)

Q9. Can this product be used for mouse iPS cells?

A9. Currently, there is no data to support culture of mouse iPS cells using iMatrixTM-511.

Q10. What is the difference between iMatrixTM-511 and Matrigel?

A10. Matrigel contains laminin-111 from mouse EHS sarcoma.
It also contains molecules other than laminin.
iMatrixTM-511 is a recombinant protein purified from laminin 511-E8 fragment expressed in CHO-S cells with high purity.
iMatrixTM-511 silk is a recombinant protein in which the laminin 511-E8 fragment is highly purified by silkworm-produced cocoons.
Human ES cells and iPS cells are known to adhere to laminin-511 through cell membrane receptors (especially a6b1 integrin).
Human ES cells and iPS cells are known to exhibit high adhesion activity to laminin-511.
This makes it possible to pass iPS cells into single cells and pass them with iMatrixTM-511 / iMatrixTM-511 silk.
In Miyazaki et al. (Nature communications, 3 (1236), 1-10, 2012), the expansion efficiency of the number of cells after 5 passages (after 30 days) is about 200 times higher than that of Matrigel.