Join us at Booth #538 to poke, prod and probe our brains on all things iMatrix™ and laminin related. Or learn what cutting edge things are being done with our products at our Innovation Showcase. Feed your mind and your belly as Dr. Hayashi and Dr. Taniguchi present their findings during our showcase, complimentary lunch is guaranteed for registered attendees. Please register by Saturday, June 15th, as seating is limited, and so we can best meet any dietary restrictions (It is now past the deadline, but you may still register although we may be unable to meet your dietary restrictions). By registering you are also automatically entered into a prize drawing held at end of the Innovation Showcase.
26-29 June, 2019
Los Angeles Convention Center
Los Angeles, CA 90015
ISSCR is the world's largest stem cell focused meeting with over 4000 attendees including researchers, key opinion leaders, speakers, exhibitors, sponsors, and media. This year the Opening Keynote is being given by Nobel Laureate Dr. Shinya Yamanaka, MD, PhD, Center for iPS Cell Research & Application (CIRA), Kyoto University, Japan and Gladstone Institutes, USA. Dr. Yamanaka and his team uses iMatrix™ products in their research and it is also used to produce clinical grade stem cell banks. ISSCR brings together seniors scientist and budding researchers to discover, share, and network with leading innovators in the stem cell community.
For more information please visit the ISSCR website here.
Time: Friday, June 28th, 2019: 11:30AM-12:30PM
Location: Concourse F - Level 1
Laminin E8 Technology for Stem Cell Proliferation and Differentiation:
From Molecular Mechanism to Clinical Application
Dr. Ryuhei Hayashi, Professor, Graduate School of Medicine, Osaka University
Dr. Yukimasa Taniguchi, Assistant Professor, Institute for Protein Research, Osaka University
Dr. Ryuhei Hayashi’s technique for differentiating human pluripotent stem cells into ocular cells has recently been approved by Japan’s Ministry of Health, Labour and Welfare for cell therapy treatment of corneal epithelial stem cell deficiency. His group has developed the culture system for self-formed ectodermal autonomous multi-zone (SEAM), cell colonies that are arranged as four concentric areas that mimic the spatiotemporal development of the whole eye. This was accomplished with laminin E8, a recombinant laminin fragment, as the culture substrate. The group has since recently uncovered the specific isoforms of laminin E8 responsible for the induction of pluripotent stem cells to the corneal epithelium, the retina, and the epithelium of the lens. The outcome of their research underscores the importance of selecting appropriate laminin isoforms for cell culturing.
The binding affinity of substrates and integrins determines the nature of expanded human pluripotent stem cell colonies in terms of cell motility, cell-cell interactions and cell density. Currently, how binding between laminin and integrin is achieved is poorly understood. Dr. Yukimasa Taniguchi will present his group’s latest findings on the molecular mechanism of laminin E8-integrin interactions underlying stem cell culturing.
Dr. Ryuhei Hayashi
Professor, Graduate School of Medicine, Osaka University
Dr. Ryuhei Hayashi graduated with his bachelor’s degree in Biochemistry from Kobe University where he further went to pursue his Master’s in Biochemistry. He then went on to Osaka University Graduate School of Medicine where he studied Ophthalmology and Stem Cell Biology and subsequently received his Ph.D. and graduated as a Doctor of Medicine in Ophthalmology from Tohoku University Graduate School of Medicine. Dr. Hayashi has won numerous awards including the Japan Cornea Society Uchida and Young Investigator Awards and the Japanese Ophthalmological Society Young Investigator Award to name just a few. Dr. Hayashi is currently a Professor of Stem Cells and Applied Medicine at Osaka University Graduate School of Medicine.
Dr. Yukimasa Taniguchi
Assistant Professor, Institute for Protein Research, Osaka University
Dr. Yukimasa Taniguchi graduated with his bachelor’s degree in Department of Biology, Faculty of Science from Kyushu University. After he went to pursue his Master’s in Graduate School of Science, Osaka University, he received his Ph.D. in Biology for Osaka University with his thesis on “Studies on the Mechanism of the Laminin-Integrin Interaction”. He then went on the work as a Post-Doctoral Fellow at the Institute for Protein Research at Osaka University. He is now an Assistant Professor at the Institute for Protein Research at Osaka University and is also a Project Leader at Matrixome, Inc.
Scraper-Free Detachment Method Using EDTA without Trypsin for Human Induced Pluripotent Stem Cells Cultured on Laminin-511E8
Dr. Fumi Ebisu, Project Leader, R&D, Matrixome Inc.
Poster #: W-3235
Session: Poster I – Odd
Date: June 26, 2019
Time: 18:30 (6:30 PM)
Recombinant E8 fragment of laminin-511 (LM511E8) is used in the culturing of human pluripotent stem cells (hPSCs) because it sustains long-term single cell passaging, preserves pluripotency and maintains cells in an undifferentiated state. LM511E8 is a truncated form of laminin-511, and its ability to offer these features comes from its binding activity with integrin α6β1, an isoform predominantly expressed on hPSCs. However, because of their strong interaction with each other, trypsinization followed by scraping is required to harvest the cultured cells. Nevertheless, cell scraping causes mechanical damage to the cells, reducing cell viability. In addition, cell scraping cannot be used when cells are cultured on multi-layer flasks or in automated cell culture systems. In this study we aim to develop a scraper-free cell detachment method for human induced pluripotent stem cells (hiPSCs) cultured on LM511E8. By referring to the protocols available to date, we examined whether incubation of hiPSCs with 5 mM EDTA/PBS(-) at 37˚C for 15 min enabled to detach the cells with high efficiency. The inclusion of trypsin to EDTA/PBS(-) was also tested to see if there were any additional gain to cell detachment efficiency. We found that more than 95% of hiPSCs were detached without compromising cell viability when they were incubated with 5 mM EDTA/PBS(-) alone. Surprisingly, the addition of trypsin decreased the detachment efficiency. This decrease was rescued when enough trypsin inhibitor was added to neutralize the enzyme, therefore the lowered detachment rate must be a result of its proteolytic activity, suggesting an ill-defined mechanism operating in hiPSCs to render them less susceptible for cell detachment by depletion of divalent cations.
Dr. Fumi Ebisu graduated with her bachelor’s degree in Clinical Laboratory Science from Eastern Michigan University where she further went to pursue her Master’s in Chemistry. Later on, she received her Ph.D. in Neuroscience from University of Michigan thanks to her thesis on “Macrophage migration inhibitory factor acts as a neurotrophin in the developing inner ear”. She then went on to work as a Post-Doctoral fellow at the Otolaryngology Department at Kyoto University. She is now a visiting research scholar at the Institute for Protein Research at Osaka University and the Project Leader at Matrixome, Inc.