iMatrix-511/-511 silk Pre-mix method (WITHOUT PRE-COATING)

【 Download 】Protocol, CATALOG

Advantages of using iMatrix-511/-511 silk


 

  • No pre-coating required
  • Requires half iMatrix-511 /-511 silk
  • No loss of cells and culture dishes

● No pre-coating required

● Requires half iMatrix-511 /-511 silk

The pre-mixed method markedly reduced the amount of substrate needed for the maximum adhesion of hPSCs compared with the pre-coat method.

You can obtain the same results using half iMatrix-511/-511 silk.

● No loss of cells and culture dishes

Have you had difficulties with cell passages?
– The number of cells are not enough to the number of dishes.
– The number of cells are too many for the number of dishes.

【 Reference 】
Miyazaki et al. Efficient adhesion culture of human pluripotent stem cells using laminin fragments in an uncoated manner.
Scientific Reports, 7, 41165, (2017).

【 Press release 】
Below above Kyoto University WEB site.
URL:http://www.kyoto-u.ac.jp/ja/research/research_results/2016/170130_1.html